Author(s): LU Peng*, LIU Lijun, and WANG Jian
In order to improve the production of L-isoleucine, the seed culture medium and the fermentation culture medium were examined by L-isoleucine
producing strain Brevibacterium flavum KM011(Met-+Ethr+α-ABr+LysL+AECr). Under the optimizing seed culture medium and fermentation culture
medium, L-isoleucine productions were increased to 9.493 g/L and 13.35 g/L in shake flasks, respectively. The strain KM011 got a final titer of 35.26
g/L under the optimal conditions by using 5L fermenter.