Introduction: Over the years improvement has been made on the staining techniques for the morphological analysis of sperm cells ranging from the normal eosin and nigrosine combination to other more simple or complex single staining technique.
Aim: Analysis of spermatozoa morphology using hibiscus flower extract as counter stain for abnormalities and viability studies towards future applications in assisted reproductive science.
Method: 22 wister rats and rabbits sperm cells where stained to analyze their morphology, using both the conventional technique and the hibiscus flower extract to determine the reproducibility of this improved staining technique.
Results: From the staining technique the morphology of the sperm cells where properly noticeable from the head, body and tail which is represented in the figures 1-4.
Conclusion: In a third world country this hibiscus plants are accessible stains for quick and accurate morphological observation of wister rats and rabbit spermatozoa as an intervention tool in absence of complex staining techniques and since it is natural may have little or no effect on sperm cells.