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Amino acid Actual weight (mg) Calculated weight via deriv. GC/MS (mg) Recovery (%)  Calculated weight after acid hydrolysis and deriv. GC/MS (mg) Recovery (%) 
Mean ± 95% CI Mean ± 95% CI Mean ± 95% CI Mean ± 95% CI Mean ± 95% CI
(n=3) (n=9) (n=9) (n=9) (n=9)
         
         

Ala*

0.12 ± 0.01

0.14 ± 0.02

118 ± 2

0.13± 0.03

105* ± 2

Gly

0.13 ± 0.02

 

0.12 ± 0.02

97 ± 2

0.07 ± 0.01

 

72 ± 1

Val*

0.078 ±0.004

0.08 ± 0.01

110 ± 1

0.08 ± 0.01

109* ± 7

Leu*

0.147 ±0.002

0.14 ± 0.01

93 ± 6

0.16 ± 0.02

105* ± 3

Ile*

0.19 ± 0.02

0.17 ± 0.03

89 ± 3

0.18 ± 0.04

88* ± 3

Pro*

0.16 ± 0.02

0.14 ± 0.02

99 ± 4

0.15 ± 0.02

108* ± 1

Ser

0.16 ± 0.03

 

0.18 ± 0.04

 

103 ± 4

0.12 ± 0.04

70 ± 4

Thr

0.21 ± 0.04

0.21 ± 0.03

 

98 ± 2

0.15 ± 0.03

 

75 ± 2

Asp*

0.16 ± 0.03

0.16 ± 0.04

105 ± 3

0.19 ± 0.02

103* ± 1

Glu*

0. 18 ± 0.02

0.21± 0.04

106 ± 2

0.16 ± 0.03

92* ± 1

Phe*

0.22 ± 0.01

0.22 ± 0.03

103 ± 1

0.19 ± 0.04

91* ± 2

Lys*

0.18 ± 0.02

0.21 ± 0.02

111 ± 1

0.13 ± 0.01

93* ± 5

Tyr

0.21 ± 0.02

0.23 ±0.02

 

110 ± 1

0.14 ± 0.1

73 ± 4

Cyt

0.29 ± 0.03

 

0.3 ± 0.1

102 ± 3

   

Average recovery (%)

   

103

 

99*

* Average of the best-recovered amino acids: Ala, Leu, Phe, Asp, Glu, Val, Ile, Pro, and Lys.
Table 5. Amino acid recovery (%) calculated from single amino acids.