Enliven: Bio analytical Techniques | Open Access Journals | Open Access Articles
  Retention time (min) Regression line (n=3) Limit of detection (µmol/L) Limit of 
Amino acid quantitation
  R2 ± 95% CI Slope ± 95% CI  Intercept ± 95% CI (µmol/L)

Ala

5.19

0.991 ± 0.003

5.9 ± 0.2

0.042 ± 0.007

0.07

0.3

Gly

5.36

0.99 ± 0.01

8.7 ± 0.7

0.04 ± 0.04

0.04

0.2

Val

6.73

0.991 ± 0.003

6.9 ± 0.2

0.031 ± 0.005

0.04

0.2

Leu

7.85

0.994 ± 0.003

5.3 ± 0.1

0.03 ± 0.02

0.07

0.2

Ile

7.79

0.993 ± 0.002

6.5 ± 0.2

0.031 ± 0.005

0.04

0.1

Pro

7.84

0.995 ± 0.002

8.5 ± 0.1

0.021 ± 0.009

0.04

0.1

Ser

8.71

0.996 ± 0.009

9.8 ± 0.2

0.008 ± 0.007

0.04

0.1

Thr

9.07

0.993 ± 0.006

12.7 ± 0.7

0.04 ± 0.09

0.08

0.2

Asp

10.66

0.997 ± 0.002

15.9 ± 0.9

-0.09 ± 0.07

0.05

0.1

Glu

11.48

0.99 ± 0.01

5.03 ± 1.27

-0.176 ± 0.007

0.04

0.3

Phe

11.54

0.996 ± 0.009

9.6 ± 0.7

-0.07 ± 0.06

0.07

0.2

Lys

13.15

0.99 ± 0.02

4.5 ± 0.3

-0.11± 0.01

0.08

0.2

Tyr

13.24

0.995 ± 0.003

9.5 ± 0.5

-0.24 ± 0.09

0.08

0.2

Cyt

14.64

0.97 ± 0.01

1.3 ± 0.4

-0.2 ± 0.1

0.1

0.5

Table 4. Retention time, R2 values, calibration equation, limit of detection (LOD), and limit of quantitation (LOQ) of the derivatized amino acids using SIM mode after normalization to the internal standard, Nor.