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Calibration parameters SIM (n=14) TIC (n=14)
Absolute Area Relative Area P-value  Absolute Height Relative Height P-value Absolute Area Relative Area P-value  Absolute Height Relative Height P-value 
Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI  Mean ± 95%CI 

R2

0.95

0.993

0.001

0.94

0.97

0.002

0.95

0.97

0.01

0.95

0.97

0.02

± 0.02

± 0.003

± 0.03

± 0.01

± 0.03

± 0.02

± 0.01

± 0.02

LOD (µmol/L)

0.09 ± 0.04

0.06 ± 0.02

0.04

0.13 ± 0.09

0.07 ± 0.01

0.03

0.14 ± 0.08

0.07 ± 0.01

0.01

0.09 ± 0.04

  

0.07 ± 0.02

0.04

Min LOD

0.05

0.04

 

0.05

0.05

 

0.06

0.07

 

0.08

0.07

 

Max LOD

0.3

0.1

 

0.9

0.1

 

0.9

0.1

 

0.9

0.1

 

Table 2. The comparison of absolute peak area, relative peak area, absolute peak height, and relative peak height in SIM and TIC modes, the mean is the average of the 14 amino acids. A matched-sample t-test was used to determine the p-values.