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EDCs Time, min SRM Transitions Cone (V) Coll. (eV)
E2 0.00 - 12.9 287 → 171 50 40
287 → 145 50 40
E3 12.9 - 21.1 271 → 145 50 40
271 → 183 50 40
DES 12.9 - 21.1 267 → 222 30 35
267 → 237 30 50

Table 4. Time scheduled SRM conditions used in LC-MS-ESI (negative mode) analysis of targeted analytes

SRM, Selected reaction monitoring; LC-MS-ESI, Liquid chromatograph - mass spectrometer - Electron spray ionization; EDCs, Endocrine disrupting chemicals; E2, 17ß-estradiol; E3, Estriol; DES, Diethylstilbestrol;