Ultra High performance liquid chromatography is a simple, sensitive and accurate gradient Reverse Phase Chromatography. It is a highly sophisticated instrument and was developed for the determination of Dipyridamole in pharmaceutical dosage form. The effective separation was achieved on phenomenex-C18; 4×250 mm, 5μm. The mixture of buffer and methanol which used as a mobile phase was taken in the ratio of 90:10 v/v. The buffer was prepared as 0.75 g of potassium dihydrogen orthophosphate in 500 ml of HPLC deionised water and the pH 7.2 buffer was adjusted with 2.5% of potassium hydroxide solution. The flow rate of the mobile phase was 1 ml/min and total elution time was 5 min. The UV detection wavelength was carried out at 291 nm and experiments were conducted at 40°C. The developed method was validated in terms of system linearity, precision, accuracy, repeatability, selectivity, and robustness, limit of detection and limit of quantification as per ICH guidelines. The results collectively demonstrated that the proposed method is selective.
Author(s): Nalini Kanta Sahoo