The aim of our study to comparative the suitability of chromatographic techniques such as thin layer chromatography (TLC)-densitometry and high performance liquid chromatography (HPLC) for the analysis of biogenic amines in fish and meat products was carried out. The recovery percent of the tested biogenic amines spiked to sausage and smoked herring samples was 92.0-102.2% and 94.5-100.3 % for HPLC versus 86.2-98.5 % and 88.0- 98.0 %for the recommended one-dimensioned TLC, respectively. It could be observed that there were not significant differences in the recovery percentages of tryptamine, β -phenylethylamine, putrescine, histamine and tyramine spiked to either sausage sample or smoked herring sample between the tested tow analytical methods. Therefore, the TLC-densitometry was found to be rapid and less expensive. In addition, this method is facile, fast, routine, cost effective and suitable method for the analysis of wide range eight biogenic amines and simultaneous screening of several samples at a time.
Author(s): M.M. Deabes, Naguib, Kh, Ayesh, A M, El-Damaty EM, and Rowayshed GH