Background:
Quantitation of bacterial density in animal tissues is commonplace in pre-clinical antibacterial drug discovery and development research. Routinely used
tissue homogenization methods are labor intensive, time consuming processes that can lead to repetitive motion related injuries to laboratory personnel.
Findings:
In this study, we compared our standard method of tissue homogenization, mechanical tissue grinders, to the Mini Beadbeater 96 for the homogenization
of murine lung and thigh tissue from standard neutropenic infection models. The Mini Beadbeater 96 proved to be an effective method for homogenizing
murine lung and thigh tissues for subsequent bacterial quantitation, with results comparable to those obtained with mechanical tissue grinders.
Conclusion:
This method proved to be a practical, high throughput, time efficient, cost effective, ergonomically friendly method for tissue homogenization.
Author(s): Lucinda M Lamb, Jennifer Tabor-Rennie, Jared L Crandon, and David P Nicolau